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How do you radioactively label a DNA fragment?

To radioactively label a DNA fragment for use as a probe, one of the incorporated nucleotides provided in the reaction is radiolabeled on the alpha phosphate position. The translated nick can be sealed by DNA ligase.

How are radioactively labeled DNA probes used in autoradiography?

Then, radioactively labeled probe is mixed with the sample DNA. Then a washing is given to remove any non-hybridized probe from the mixture. The radio­labeled probes are then detected (in solution or within a solid sample) by autoradiography.

What are radioactively labeled nucleic acids used for in hybridization?

These radioactively labeled nucleic acids are then used as probe in radioactive hybridization procedures. In a standard radioactive hybridization detection system, the sample DNA is first bound to a solid support. Then, radioactively labeled probe is mixed with the sample DNA.

What is the difference between radioactive and non-radioactive DNA probes?

Radioactive probes are the single-stranded DNA or RNA sequences labeled with radioactive isotopes, while nonradioactive probes are the single-stranded DNA or RNA sequences labeled with a chemical tag. So, this is the key difference between radioactive and nonradioactive probes.


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