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What are the radioactive labeling techniques?

This chapter discusses the radioactive labeling techniques. Radioactive labeling techniques are of central importance in the investigation of the biosynthesis of glycosylated proteins for detection of intermediates and identification of the subcellular sites of steps in biosynthesis, measuring rates of synthesis, ...

What is radiolabeling?

In the latter case, the labeling is called radiolabeling . In isotopic labeling, there are multiple ways to detect the presence of labeling isotopes; through their mass, vibrational mode, or radioactive decay.

How many radioactive labels are there in a drug?

Generally, only one radioactive label is incorporated into a drug because drug metabolism typically leads to a modified structure with little fragmentation of the molecule. If you go back far enough in a synthesis, however, it is possible to synthesize a drug with several carbon atoms radioactively labeled.

What are the nuclides used in isotopic labeling?

The nuclides used in isotopic labeling may be stable nuclides or radionuclides. In the latter case, the labeling is called radiolabeling . In isotopic labeling, there are multiple ways to detect the presence of labeling isotopes; through their mass, vibrational mode, or radioactive decay.


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