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What are radioactively labeled nucleic acids used for in hybridization?

These radioactively labeled nucleic acids are then used as probe in radioactive hybridization procedures. In a standard radioactive hybridization detection system, the sample DNA is first bound to a solid support. Then, radioactively labeled probe is mixed with the sample DNA.

How do you radioactively label a DNA fragment?

To radioactively label a DNA fragment for use as a probe, one of the incorporated nucleotides provided in the reaction is radiolabeled on the alpha phosphate position. The translated nick can be sealed by DNA ligase.

How are radioactively labeled DNA probes used in autoradiography?

Then, radioactively labeled probe is mixed with the sample DNA. Then a washing is given to remove any non-hybridized probe from the mixture. The radio­labeled probes are then detected (in solution or within a solid sample) by autoradiography.

How do you label DNA and RNA?

DNA and RNA labeling techniques Oligonucleotides can be labeled at either the 3' or the 5' end. Using polynucleotide kinase and ATP-gamma- 32 P, the 5' end is labeled. Using terminal transferase and deoxynucleotide triphosphate labeled on the alpha phosphate, the 3' end is labeled.


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